Why Accurate Math Matters
Imprecise reconstitution undermines reproducibility. A 10% error in concentration means a 10% error in every subsequent measurement. This guide covers the essential calculations and provides reference values for common peptide/volume combinations.
The Basic Formula
Concentration (mg/mL) = Peptide Mass (mg) ÷ Water Volume (mL)
Example: Dissolving a 5mg vial in 2mL of bacteriostatic water yields a 2.5 mg/mL solution.
Converting to mcg per Insulin Unit
Insulin syringes are marked in "units" (IU), where 100 units = 1mL. To calculate mcg per unit:
mcg per unit = Concentration (mcg/mL) ÷ 100
For a 2.5 mg/mL solution: 2,500 mcg/mL ÷ 100 = 25 mcg per unit. So 10 units on the syringe = 250 mcg.
Common Reference Values
| Peptide | Volume Added | Concentration | 10 units = |
|---|---|---|---|
| BPC-157 5mg | 2mL | 2.5 mg/mL | 250 mcg |
| Semaglutide 5mg | 2.5mL | 2 mg/mL | 200 mcg |
| TB-500 5mg | 1mL | 5 mg/mL | 500 mcg |
| Ipamorelin 5mg | 2mL | 2.5 mg/mL | 250 mcg |
| CJC-1295 DAC 2mg | 2mL | 1 mg/mL | 100 mcg |
| GHK-Cu 50mg | 3mL | 16.7 mg/mL | 1,667 mcg |
Tips for Accuracy
- Use the smallest syringe that accommodates your volume — 0.5mL syringes have finer graduation marks than 1mL
- Draw slowly and watch for air bubbles — flick the syringe gently and push out trapped air before adding to the peptide vial
- Mark the desired volume on the syringe barrel before drawing
- Record the exact volume added and date on the vial label
For instant calculations, use our online Reconstitution Calculator — enter peptide mass and desired concentration, and it calculates the water volume automatically.
For research purposes only.