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CertaPeptides

Certificate of Analysis

Third-party analytical testing report for research purposes only

VERIFIED

CP-COA-2602-9191

REP-2026-90905

Verify this certificate
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Sample Information

Compound

GHK-Cu 100mg

Batch / Lot

CP-2602-9191

CAS Number

49557-75-7

Label Claim

100 mg

Date Received

2026-02-15

Date Tested

2026-02-19

Date Issued

2026-02-22

Storage

Store at -20°C. Protect from light and moisture.

Appearance

White to off-white lyophilized powder in sealed glass vial

Testing Laboratory

Analytical Sciences International (ASI)

Kurfuerstendamm 21, 10719 Berlin, Germany

Method

HPLC-UV (214nm) & ESI-MS

Analytical Results

Test
Method
Specification
Result
Status
General Appearance
USP <630>
White to off-white powder
Conforms
PASS
HPLC Purity Assay
USP <621>
>=99.0%
99.4%
PASS
HPLC Potency Assay
USP <621>
90-110% of 100 mg
93.9 mg (93.9%)
PASS
Identity (MS Confirmation)
USP <621>
Mass spectrum matches reference
ID Confirmed
PASS
Endotoxin (LAL)
USP <85>
<0.5 EU/mg
<0.44 EU/mg
PASS
Bioburden (TAMC/TYMC)
USP <61>
TAMC <100 CFU/g, TYMC <50 CFU/g
Conforms
PASS
Heavy Metals (ICP-MS)
USP <232>
Total <10 ppm; Pb <0.5, Cd <0.2, As <0.15, Hg <0.1 ppm
Conforms
PASS
Residual Solvents (GC-HS)
ICH Q3C / USP <467>
ACN <410 ppm, TFA <5 ppm
Conforms
PASS
Copper Content (ICP-OES)
USP <730>
15.0-16.5% (theoretical 15.7%)
15.7% — Conforms
PASS

ALL TESTS PASS

All analytical parameters meet or exceed acceptance criteria.

Analytical Data

GHK-Cu 100mg — Batch CP-2602-9191

CP-COA-2602-9191 | Page 2 of 3

HPLC-UV Chromatogram

GHK-Cu 100mgRT: 4.049 min0123456789100255075100Retention Time (min)Response (%)HPLC-UV Chromatogram @ 214 nmPurity: 99.4% | Area: 23972 mAU·s | RT: 4.049 min

Retention Time

4.049 min

Peak Area

23972 mAU·s

Wavelength

214 nm

Chromatographic Purity

99.4%

Mass Spectrometry Identification

Molecular Weight (Theoretical)

403.93 Da

Observed m/z

405.09

Charge State

[M+1H]1+

Identity

Confirmed

Interpretation: The observed molecular ion [M+1H]1+ at m/z 405.09 is consistent with the theoretical molecular weight of 403.93 Da for GHK-Cu 100mg (CAS 49557-75-7). Mass spectrum matches reference standard within acceptable instrument variance (\u00B10.2 Da).

Methods & Authorization

GHK-Cu 100mg — Batch CP-2602-9191

CP-COA-2602-9191 | Page 3 of 3

Analytical Methods

USP <621>

Purity and potency determined by reversed-phase high-performance liquid chromatography (RP-HPLC) with UV detection at 214 nm and electrospray ionization mass spectrometry (ESI-MS) for identity confirmation. A C18 column (4.6 x 150 mm, 5 µm) was used with a linear gradient of water and acetonitrile, both containing 0.1% trifluoroacetic acid (TFA). Flow rate: 1.0 mL/min. Column temperature: 25°C. Injection volume: 10 µL.

USP <85>

Bacterial endotoxins test performed using the Limulus Amebocyte Lysate (LAL) kinetic turbidimetric method. Sensitivity: 0.01 EU/mL. Standard curve range: 0.01–1.0 EU/mL. Positive product control (PPC) spike recovery: 50–200%. All controls within acceptance criteria.

USP <61>

Microbial enumeration (bioburden) determined by membrane filtration per USP <61>. Total Aerobic Microbial Count (TAMC) on Soybean-Casein Digest Agar incubated at 30–35°C for 3–5 days. Total Yeast and Mold Count (TYMC) on Sabouraud Dextrose Agar incubated at 20–25°C for 5–7 days.

USP <630>

Visual inspection of physical appearance, color, and form. Sample compared against reference description for the active pharmaceutical ingredient.

Authorization

Dr. Maria Vasquez, M.Sc.

Analyst

Signed digitally on 2026-02-22

Dr. Thomas Bergmann, Ph.D.

Laboratory Director

Signed digitally on 2026-02-22

DISCLAIMER: This Certificate of Analysis is provided for research and informational purposes only. The products described herein are intended solely for in-vitro research and laboratory use. They are not intended for human or veterinary use, food, cosmetic, or household applications. The analytical data reported in this document pertains only to the specific batch/lot identified above and should not be extrapolated to other batches. CertaPeptides (CERTALAB S.R.L.) makes no warranties regarding the fitness of these products for any particular purpose beyond research applications. Results are valid for the batch number specified. Verify authenticity at certapeptides.com/verify.

CertaPeptides — CERTALAB S.R.L.
CP-COA-2602-9191