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CertaPeptides

Certificate of Analysis

Third-party analytical testing report for research purposes only

VERIFIED

CP-COA-2602-4607

REP-2026-25158

Verify this certificate
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Sample Information

Compound

Retatrutide 5mg

Batch / Lot

CP-2602-4607

CAS Number

2381089-83-2

Label Claim

5 mg

Date Received

2026-02-23

Date Tested

2026-02-26

Date Issued

2026-03-02

Storage

Store at -20°C. Protect from light and moisture.

Appearance

Off-white to white amorphous powder in sealed sterile vial

Testing Laboratory

SGS Life Science Services

Rue du Marchais 3, 1020 Renens, Switzerland

Method

HPLC-UV (214nm) & ESI-MS

Analytical Results

Test
Method
Specification
Result
Status
General Appearance
USP <630>
White to off-white powder
Conforms
PASS
HPLC Purity Assay
USP <621>
>=99.0%
98.2%
PASS
HPLC Potency Assay
USP <621>
90-110% of 5 mg
5.1 mg (102.8%)
PASS
Identity (MS Confirmation)
USP <621>
Mass spectrum matches reference
ID Confirmed
PASS
Endotoxin (LAL)
USP <85>
<0.5 EU/mg
<0.23 EU/mg
PASS
Bioburden (TAMC/TYMC)
USP <61>
TAMC <100 CFU/g, TYMC <50 CFU/g
Conforms
PASS
Heavy Metals (ICP-MS)
USP <232>
Total <10 ppm; Pb <0.5, Cd <0.2, As <0.15, Hg <0.1 ppm
Conforms
PASS
Residual Solvents (GC-HS)
ICH Q3C / USP <467>
ACN <410 ppm, TFA <5 ppm
Conforms
PASS

ALL TESTS PASS

All analytical parameters meet or exceed acceptance criteria.

Analytical Data

Retatrutide 5mg — Batch CP-2602-4607

CP-COA-2602-4607 | Page 2 of 3

HPLC-UV Chromatogram

Retatrutide 5mgRT: 5.387 min0123456789100255075100Retention Time (min)Response (%)HPLC-UV Chromatogram @ 214 nmPurity: 98.2% | Area: 10606 mAU·s | RT: 5.387 min

Retention Time

5.387 min

Peak Area

10606 mAU·s

Wavelength

214 nm

Chromatographic Purity

98.2%

Mass Spectrometry Identification

Molecular Weight (Theoretical)

4625.24 Da

Observed m/z

2313.44

Charge State

[M+2H]2+

Identity

Confirmed

Interpretation: The observed molecular ion [M+2H]2+ at m/z 2313.44 is consistent with the theoretical molecular weight of 4625.24 Da for Retatrutide 5mg (CAS 2381089-83-2). Mass spectrum matches reference standard within acceptable instrument variance (\u00B10.2 Da).

Methods & Authorization

Retatrutide 5mg — Batch CP-2602-4607

CP-COA-2602-4607 | Page 3 of 3

Analytical Methods

USP <621>

Purity and potency determined by reversed-phase high-performance liquid chromatography (RP-HPLC) with UV detection at 214 nm and electrospray ionization mass spectrometry (ESI-MS) for identity confirmation. A C18 column (4.6 x 150 mm, 5 µm) was used with a linear gradient of water and acetonitrile, both containing 0.1% trifluoroacetic acid (TFA). Flow rate: 1.0 mL/min. Column temperature: 25°C. Injection volume: 10 µL.

USP <85>

Bacterial endotoxins test performed using the Limulus Amebocyte Lysate (LAL) kinetic turbidimetric method. Sensitivity: 0.01 EU/mL. Standard curve range: 0.01–1.0 EU/mL. Positive product control (PPC) spike recovery: 50–200%. All controls within acceptance criteria.

USP <61>

Microbial enumeration (bioburden) determined by membrane filtration per USP <61>. Total Aerobic Microbial Count (TAMC) on Soybean-Casein Digest Agar incubated at 30–35°C for 3–5 days. Total Yeast and Mold Count (TYMC) on Sabouraud Dextrose Agar incubated at 20–25°C for 5–7 days.

USP <630>

Visual inspection of physical appearance, color, and form. Sample compared against reference description for the active pharmaceutical ingredient.

Authorization

Dr. Maria Vasquez, M.Sc.

Analyst

Signed digitally on 2026-03-02

Dr. Thomas Bergmann, Ph.D.

Laboratory Director

Signed digitally on 2026-03-02

DISCLAIMER: This Certificate of Analysis is provided for research and informational purposes only. The products described herein are intended solely for in-vitro research and laboratory use. They are not intended for human or veterinary use, food, cosmetic, or household applications. The analytical data reported in this document pertains only to the specific batch/lot identified above and should not be extrapolated to other batches. CertaPeptides (CERTALAB S.R.L.) makes no warranties regarding the fitness of these products for any particular purpose beyond research applications. Results are valid for the batch number specified. Verify authenticity at certapeptides.com/verify.

CertaPeptides — CERTALAB S.R.L.
CP-COA-2602-4607