Research-grade compound. Laboratory use only. Not intended for human or animal use, ingestion, or injection. No medical claims are made or implied.
Bacteriostatic Water
USP-grade bacteriostatic water (3ml) with 0.9% benzyl alcohol. Sterile, endotoxin-tested. For peptide reconstitution.
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For research purposes only. Not for human or veterinary use.
Product details
Bacteriostatic water is sterile water containing 0.9% benzyl alcohol (9mg per ml) as a bacteriostatic preservative. It is the standard reconstitution medium for research peptides that will be stored after preparation and used over multiple sessions. The benzyl alcohol does not sterilize -- it does not kill existing contamination -- but it prevents bacterial growth from needle punctures and environmental exposure, extending the usable window of a reconstituted peptide solution to approximately 28-30 days when kept refrigerated.
When to Use Bacteriostatic Water
Any peptide protocol that involves reconstituting a vial and using it for multiple doses over days or weeks should use bacteriostatic water. The alternative -- sterile water -- has no preservative action and is appropriate only for single-use, immediate-consumption reconstitutions. In practice, most research protocols involving peptides dosed repeatedly in animal studies or kept as working stocks in cell culture work default to bacteriostatic water for this reason.
One consideration: benzyl alcohol is biologically active at higher concentrations. At 0.9% in a reconstitution vial, the contribution to a typical in vivo dose or in vitro working concentration is negligible. However, if you are performing a sensitive cell viability assay or working with very high volumes of reconstituted solution per experiment, it is worth calculating the final benzyl alcohol concentration in the experimental medium and comparing to known cytotoxicity thresholds (generally above 0.1% in culture).
Specifications
| Parameter | Value |
|---|---|
| Volume | 10ml vial |
| Preservative | 0.9% benzyl alcohol (9mg/ml) |
| pH | 4.5 - 7.0 |
| Sterility | Sterile filtered |
| Endotoxins | <0.5 EU/ml |
Storage
Store unopened vials at room temperature or at 4°C, away from light. Once the vial septum has been punctured, refrigerate at 2-8°C and use within 28 days. Label with opening date. Discard if the solution becomes cloudy, discolored, or if visible particulates appear -- all of which indicate contamination despite the preservative. Do not freeze bacteriostatic water as this can cause precipitation.
Can bacteriostatic water be used for every type of peptide?
For the vast majority of synthetic research peptides, yes. The main exceptions are highly acid-sensitive or base-sensitive sequences where the pH 4.5-7.0 range of bacteriostatic water could affect stability, and some labile peptides where even the small amount of benzyl alcohol might interfere with the assay. For most standard peptides -- BPC-157, TB-500, GLP-1 analogues, growth hormone peptides, CJC-1295, Ipamorelin -- bacteriostatic water is fully compatible and is the recommended reconstitution medium.
What volume should be used to reconstitute a typical research peptide?
This depends entirely on your working concentration needs. A common approach: add enough bacteriostatic water so that each 10 units (0.1ml) on a U-100 insulin syringe represents a convenient dose increment. For example, reconstituting 2mg in 2ml gives 1mg/ml -- 100 mcg per 10-unit mark. Reconstituting 5mg in 2.5ml gives 2mg/ml -- 200 mcg per 10 units. Calculate your target dose, decide what volume increment is practically measurable with your syringe, then work backward to your reconstitution concentration.
Bacteriostatic water is supplied for laboratory research use only. For research purposes only.