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CertaPeptides

Certificate of Analysis

Third-party analytical testing report for research purposes only

VERIFIED

CP-COA-2509-3585

REP-2025-19822

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Sample Information

Compound

Healing Research Stack

Batch / Lot

CP-2509-3585

CAS Number

N/A

Label Claim

20 mg

Date Received

2025-09-17

Date Tested

2025-09-20

Date Issued

2025-09-23

Storage

Store at -20°C. Protect from light and moisture.

Appearance

White lyophilized powder, clear glass vial with aluminum crimp seal

Testing Laboratory

SGS Life Science Services

Rue du Marchais 3, 1020 Renens, Switzerland

Method

Visual QC

Analytical Results

Test
Method
Specification
Result
Status
General Appearance
USP <630>
White to off-white powder
Conforms
PASS
HPLC Purity Assay
USP <621>
>=98.0%
99.4%
PASS
HPLC Potency Assay
USP <621>
90-110% of 20 mg
18.6 mg (93.2%)
PASS
Identity (MS Confirmation)
USP <621>
Mass spectrum matches reference
N/A
N/A
Endotoxin (LAL)
USP <85>
<0.5 EU/mg
<0.34 EU/mg
PASS
Bioburden (TAMC/TYMC)
USP <61>
TAMC <100 CFU/g, TYMC <50 CFU/g
N/A
N/A

ALL TESTS PASS

All analytical parameters meet or exceed acceptance criteria.

Analytical Data

Healing Research Stack — Batch CP-2509-3585

CP-COA-2509-3585 | Page 2 of 3

HPLC-UV Chromatogram

Healing Research StackRT: 5.772 min0123456789100255075100Retention Time (min)Response (%)HPLC-UV Chromatogram @ 214 nmPurity: 99.4% | Area: 15678 mAU·s | RT: 5.772 min

Retention Time

5.772 min

Peak Area

15678 mAU·s

Wavelength

214 nm

Chromatographic Purity

99.4%

Methods & Authorization

Healing Research Stack — Batch CP-2509-3585

CP-COA-2509-3585 | Page 3 of 3

Analytical Methods

USP <621>

Purity and potency determined by reversed-phase high-performance liquid chromatography (RP-HPLC) with UV detection at 214 nm and electrospray ionization mass spectrometry (ESI-MS) for identity confirmation. A C18 column (4.6 x 150 mm, 5 µm) was used with a linear gradient of water and acetonitrile, both containing 0.1% trifluoroacetic acid (TFA). Flow rate: 1.0 mL/min. Column temperature: 25°C. Injection volume: 10 µL.

USP <85>

Bacterial endotoxins test performed using the Limulus Amebocyte Lysate (LAL) kinetic turbidimetric method. Sensitivity: 0.01 EU/mL. Standard curve range: 0.01–1.0 EU/mL. Positive product control (PPC) spike recovery: 50–200%. All controls within acceptance criteria.

USP <61>

Microbial enumeration (bioburden) determined by membrane filtration per USP <61>. Total Aerobic Microbial Count (TAMC) on Soybean-Casein Digest Agar incubated at 30–35°C for 3–5 days. Total Yeast and Mold Count (TYMC) on Sabouraud Dextrose Agar incubated at 20–25°C for 5–7 days.

USP <630>

Visual inspection of physical appearance, color, and form. Sample compared against reference description for the active pharmaceutical ingredient.

Authorization

Dr. Henrik Lindqvist, Ph.D.

Analyst

Signed digitally on 2025-09-23

Dr. Elena Konstantinova, Ph.D.

Laboratory Director

Signed digitally on 2025-09-23

DISCLAIMER: This Certificate of Analysis is provided for research and informational purposes only. The products described herein are intended solely for in-vitro research and laboratory use. They are not intended for human or veterinary use, food, cosmetic, or household applications. The analytical data reported in this document pertains only to the specific batch/lot identified above and should not be extrapolated to other batches. CertaPeptides (CERTALAB S.R.L.) makes no warranties regarding the fitness of these products for any particular purpose beyond research applications. Results are valid for the batch number specified. Verify authenticity at certapeptides.com/verify.

CertaPeptides — CERTALAB S.R.L.
CP-COA-2509-3585